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New at LP3: equipment for in-solution binding affinity studies is now accessible

A Monolith X nanotemper machine with computer. Photo.

LP3 again extends its portfolio of available machines by welcoming the Monolith X (Nanotemper).

Thanks to infrastructure grants from LU, the faculties of Science and Medicine, we can now make the latest MicroScale Thermophoresis (MST) and Spectral Shift (SpS) equipment available to users.
The Monolith X allows in-depth interaction and binding studies between a labeled target and a ligand, for example to determine the dissociation constant (Kd) or the effective concentration (EC50) in a protein-protein interaction, within the nM to µM range. A wide range of molecules can be tested: proteins (even membrane proteins or large protein complexes), small molecules or ions…etc

Diagram with graph and typical values from a nanotemper run. Drawing.
Example of dose response curve from a run with the Monolith X

For more information about the system , please visit the Monolith X webpage (external homepage). Information regarding access and user fees is found in the biophysics and crystallisation section here at the LP3 website